Regulatory

Part:BBa_K2433005:Design

Designed by: Christine Kim   Group: iGEM17_British_Columbia   (2017-10-25)


traR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The first 200 or so base pairs before the traR gene on the pTiC58 were taken and we assumed that the promoter would be in this sequence.

Source

The sequence was designed on Snapgene using the sequence from https://www.ncbi.nlm.nih.gov/nuccore/AE007871.2 and we received it in a gBlock from IDT.

References

Zhu, J., Winans, S. C. (1999). Autoinducer binding by the quorum-sensing regulator TraR increases affinity for target promoters in vitro and decreases TraR turnover rates in whole cells. Biochemistry, 96, 4832.
Cook, D. M., Li, P. L., Ruchaud, F., Padden, S., Farrand, S.(1997). Ti Plasmid Conjugation is independent of vir: reconstitution of the tra functions from pTiC58 as a binary system. Journal of Bacteriology, 179-4, 1291.